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(A) Representative H&E staining and tissue compartment analysis with the top 20 enriched genes in each compartment. THBS1 was highlighted by red rectangles. Red arrow indicates dissection sites (presence of red blood cells). “L” indicates the aortic lumen. (B) Expression and distribution of COL1A1 , COL3A1 , TAGLN , and THBS1 in representative AAA tissue. (C) Immunostaining of human AAA cross-sections with anti-MYH11 and <t>anti-TSP1.</t> The yellow dashed ellipse indicates the dissection site. (D) TGFβ (TGFB) pathway activity and expression of ligands and receptors of TGFβ signaling in AAA tissue.
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(A) Representative H&E staining and tissue compartment analysis with the top 20 enriched genes in each compartment. THBS1 was highlighted by red rectangles. Red arrow indicates dissection sites (presence of red blood cells). “L” indicates the aortic lumen. (B) Expression and distribution of COL1A1 , COL3A1 , TAGLN , and THBS1 in representative AAA tissue. (C) Immunostaining of human AAA cross-sections with anti-MYH11 and anti-TSP1. The yellow dashed ellipse indicates the dissection site. (D) TGFβ (TGFB) pathway activity and expression of ligands and receptors of TGFβ signaling in AAA tissue.

Journal: bioRxiv

Article Title: Myeloid-Specific Thrombospondin-1 Deficiency Exacerbates Aortic Rupture via Broad Suppression of Extracellular Matrix Proteins

doi: 10.1101/2024.07.30.605216

Figure Lengend Snippet: (A) Representative H&E staining and tissue compartment analysis with the top 20 enriched genes in each compartment. THBS1 was highlighted by red rectangles. Red arrow indicates dissection sites (presence of red blood cells). “L” indicates the aortic lumen. (B) Expression and distribution of COL1A1 , COL3A1 , TAGLN , and THBS1 in representative AAA tissue. (C) Immunostaining of human AAA cross-sections with anti-MYH11 and anti-TSP1. The yellow dashed ellipse indicates the dissection site. (D) TGFβ (TGFB) pathway activity and expression of ligands and receptors of TGFβ signaling in AAA tissue.

Article Snippet: PFA-fixed tissue sections were blocked with normal donkey serum (10% vol/vol) and incubated at 4 °C overnight with primary antibodies anti-F4/80 (123102, BioLegend), anti-Col1a1 (NB600-408, Novus Biologicals), anti-MYH11 (ab53219, abcam), anti-TSP1 (AF3074, R&D Systems).

Techniques: Staining, Dissection, Expressing, Immunostaining, Activity Assay